Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine
Journal of Veterinary Science
;
: 817-826, 2018.
Article
in English
| WPRIM
| ID: wpr-758865
ABSTRACT
The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Birds
/
Bursa of Fabricius
/
T-Lymphocytes, Cytotoxic
/
Adjuvants, Immunologic
/
Cytokines
/
Influenza A Virus, H9N2 Subtype
/
Antibodies, Neutralizing
/
Immunity, Humoral
/
Influenza in Birds
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2018
Type:
Article
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