Effect of Puromycin Aminonucleoside on Podocyte P-Cadherin
Journal of the Korean Society of Pediatric Nephrology
;
: 79-85, 2013.
Article
in Korean
| WPRIM
| ID: wpr-75957
ABSTRACT
PURPOSE:
To test whether the expression of P-cadherin, a component of slit diaphragms between podocyte foot processes, would be altered by puromycin aminonucleoside (PAN) in a cultured podocyte in vitro.METHODS:
Rat glomerular epithelial cells (GEpC) were cultured with various concentrations of PAN. The distribution of P-cadherin was examined with a confocal microscope. Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to measure the change in P-cadherin expression.RESULTS:
This study found that P-cadherin was concentrated in the inner and peripheral cytoplasm with high concentrations of PAN under immunofluorescence views. Western blotting of GEpC revealed that PAN induced a decrease of P-cadherin in dose- and time-dependent manners. A high dose (50 microg/mL) of PAN decreased P-cadherin expression by 21.9% at 24 h (P<0.05) and 31.9% at 48 h (P<0.01) compared to those without PAN. In RT-PCR, high concentrations (50 microg/mL) of PAN also decreased P-cadherin mRNA expression, similar to protein suppression, by 23.5% at 48 h (P<0.05).CONCLUSION:
Podocytes exposed to PAN in vitro concentrated P-cadherin internally, and reduced P-cadherin mRNA and protein expression. This could explain the development of proteinuria in experimental PAN-induced nephropathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proteinuria
/
Ascorbic Acid
/
RNA, Messenger
/
Diaphragm
/
Puromycin
/
Puromycin Aminonucleoside
/
Cadherins
/
Blotting, Western
/
Fluorescent Antibody Technique
/
Cytoplasm
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Society of Pediatric Nephrology
Year:
2013
Type:
Article
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