Assessment of the Purity of Emodin by Quantitative Nuclear Magnetic Resonance Spectroscopy and Mass Balance
Natural Product Sciences
;
: 222-227, 2019.
Article
in English
| WPRIM
| ID: wpr-760569
ABSTRACT
Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Relaxation
/
Salts
/
Spectrum Analysis
/
Trace Elements
/
Magnetic Resonance Spectroscopy
/
Chromatography, High Pressure Liquid
/
Chromatography, Liquid
/
Emodin
/
Polygonaceae
/
Rhamnaceae
Language:
English
Journal:
Natural Product Sciences
Year:
2019
Type:
Article
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