Free fatty acid-induced histone acetyltransferase activity accelerates lipid accumulation in HepG2 cells
Nutrition Research and Practice
;
: 196-204, 2019.
Article
in English
| WPRIM
| ID: wpr-760610
ABSTRACT
BACKGROUND/OBJECTIVES:
Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disease triggered by epigenetic alterations, including lysine acetylation at histone or non-histone proteins, affecting the stability or transcription of lipogenic genes. Although various natural dietary compounds have anti-lipogenic effects, their effects on the acetylation status and lipid metabolism in the liver have not been thoroughly investigated. MATERIALS/METHODS:
Following oleic-palmitic acid (OPA)-induced lipid accumulation in HepG2 cells, the acetylation status of histone and non-histone proteins, HAT activity, and mRNA expression of representative lipogenic genes, including PPARγ, SREBP-1c, ACLY, and FASN, were evaluated. Furthermore, correlations between lipid accumulation and HAT activity for 22 representative natural food extracts (NExs) were evaluated.RESULTS:
Non-histone protein acetylation increased following OPA treatment and the acetylation of histones H3K9, H4K8, and H4K16 was accelerated, accompanied by an increase in HAT activity. OPA-induced increases in the mRNA expression of lipogenic genes were down-regulated by C-646, a p300/CBP-specific inhibitor. Finally, we detected a positive correlation between HAT activity and lipid accumulation (Pearson's correlation coefficient = 0.604) using 22 NExs.CONCLUSIONS:
Our results suggest that NExs have novel applications as nutraceutical agents with HAT inhibitor activity for the prevention and treatment of NAFLD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetylation
/
RNA, Messenger
/
Histones
/
Dietary Supplements
/
Histone Acetyltransferases
/
Sterol Regulatory Element Binding Protein 1
/
Lipid Metabolism
/
Lipogenesis
/
Hep G2 Cells
/
Epigenomics
Language:
English
Journal:
Nutrition Research and Practice
Year:
2019
Type:
Article
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