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Free fatty acid-induced histone acetyltransferase activity accelerates lipid accumulation in HepG2 cells
Nutrition Research and Practice ; : 196-204, 2019.
Article in English | WPRIM | ID: wpr-760610
ABSTRACT
BACKGROUND/

OBJECTIVES:

Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disease triggered by epigenetic alterations, including lysine acetylation at histone or non-histone proteins, affecting the stability or transcription of lipogenic genes. Although various natural dietary compounds have anti-lipogenic effects, their effects on the acetylation status and lipid metabolism in the liver have not been thoroughly investigated. MATERIALS/

METHODS:

Following oleic-palmitic acid (OPA)-induced lipid accumulation in HepG2 cells, the acetylation status of histone and non-histone proteins, HAT activity, and mRNA expression of representative lipogenic genes, including PPARγ, SREBP-1c, ACLY, and FASN, were evaluated. Furthermore, correlations between lipid accumulation and HAT activity for 22 representative natural food extracts (NExs) were evaluated.

RESULTS:

Non-histone protein acetylation increased following OPA treatment and the acetylation of histones H3K9, H4K8, and H4K16 was accelerated, accompanied by an increase in HAT activity. OPA-induced increases in the mRNA expression of lipogenic genes were down-regulated by C-646, a p300/CBP-specific inhibitor. Finally, we detected a positive correlation between HAT activity and lipid accumulation (Pearson's correlation coefficient = 0.604) using 22 NExs.

CONCLUSIONS:

Our results suggest that NExs have novel applications as nutraceutical agents with HAT inhibitor activity for the prevention and treatment of NAFLD.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Acetylation / RNA, Messenger / Histones / Dietary Supplements / Histone Acetyltransferases / Sterol Regulatory Element Binding Protein 1 / Lipid Metabolism / Lipogenesis / Hep G2 Cells / Epigenomics Language: English Journal: Nutrition Research and Practice Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Acetylation / RNA, Messenger / Histones / Dietary Supplements / Histone Acetyltransferases / Sterol Regulatory Element Binding Protein 1 / Lipid Metabolism / Lipogenesis / Hep G2 Cells / Epigenomics Language: English Journal: Nutrition Research and Practice Year: 2019 Type: Article