Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells
The Korean Journal of Parasitology
;
: 83-92, 2019.
Article
in English
| WPRIM
| ID: wpr-761733
ABSTRACT
Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parasites
/
Acetylcysteine
/
Ascorbic Acid
/
Retinaldehyde
/
Toxoplasma
/
Toxoplasmosis
/
Reactive Oxygen Species
/
Superoxides
/
Mitochondria
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2019
Type:
Article
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