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Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts
The Korean Journal of Parasitology ; : 217-223, 2019.
Article in English | WPRIM | ID: wpr-761749
ABSTRACT
Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts. Morphological observation revealed that panked dendritic HCECs co-cultured with amoeba cysts had changed into round shape and gradually died. Such changes were more severe in co-culture with cyst than those of co-cultivation with trophozoites. In vitro cytotoxicity assay revealed the highest cytotoxicity to HCECs in the co-culture system with amoeba cysts. A. castellanii induced the expression of IL-1α, IL-6, IL-8, and CXCL1 in HCECs. Secreted levels of IL-1α, IL-6, and IL-8 in HCECs co-cultured with both trophozoites and cysts were increased at an early incubation time (3 and 6 hr). These results suggested that cytopathic changes and pro-inflammatory cytokines release of HCECs in response to A. castellanii, especially amoebic cysts, are an important mechanism for AK development.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Vision Disorders / In Vitro Techniques / Acanthamoeba / Blindness / Cytokines / Interleukin-8 / Interleukin-6 / Coculture Techniques / Acanthamoeba castellanii / Epithelial Cells Limits: Humans Language: English Journal: The Korean Journal of Parasitology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Vision Disorders / In Vitro Techniques / Acanthamoeba / Blindness / Cytokines / Interleukin-8 / Interleukin-6 / Coculture Techniques / Acanthamoeba castellanii / Epithelial Cells Limits: Humans Language: English Journal: The Korean Journal of Parasitology Year: 2019 Type: Article