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D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation
Tissue Engineering and Regenerative Medicine ; (6): 177-189, 2019.
Article in English | WPRIM | ID: wpr-761893
ABSTRACT

BACKGROUND:

Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (D-RADA16-RGD sequence containing all D-amino acids is Ac-RADARADARADARADARGDS-CONH2) shows admirable bioactivity for both cell culture and bone regeneration. Here, we describe the fabrication of a favorable biomaterial material (nHA/PA66/D-RADA16-RGD).

METHODS:

Proteinase K and circular dichroism spectroscopy were employed to test the stability and secondary structural properties of peptide D-RADA16-RGD respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the surface of these materials. Confocal laser scanning (CLS), cell counting kit-8 tests (CCK-8), alizarin red S staining, cell immunofluorescence analysis and Western blotting were involved in vitro. Also biosafety and bioactivity of them have been evaluated in vivo.

RESULTS:

Proteinase K and circular dichroism spectroscopy demonstrated that D-RADA16-RGD in nHA/PA66 was able to form stable-sheet secondary structure. SEM and TEM showed that the D-RADA16-RGD material was 7–33 nm in width and 130–600 nm in length, and the interwoven pore size ranged from 40 to 200 nm. CLS suggests that cells in nHA/PA66/D-RADA16-RGD group were linked to adjacent cells with more actin filaments. CCK-8 analysis showed that nHA/PA66/D-RADA16-RGD revealed good biocompatibility. The results of Alizarin-red S staining and Western blotting as well as vivo osteogenesis suggest nHA/PA66/D-RADA16-RGD exhibits better bioactivity.

CONCLUSION:

This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Sincalide / Spectrum Analysis / Bone Regeneration / In Vitro Techniques / Actin Cytoskeleton / Microscopy, Electron, Scanning / Cell Count / Blotting, Western / Fluorescent Antibody Technique Language: English Journal: Tissue Engineering and Regenerative Medicine Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Sincalide / Spectrum Analysis / Bone Regeneration / In Vitro Techniques / Actin Cytoskeleton / Microscopy, Electron, Scanning / Cell Count / Blotting, Western / Fluorescent Antibody Technique Language: English Journal: Tissue Engineering and Regenerative Medicine Year: 2019 Type: Article