Induction of Melanoma Cell-Selective Apoptosis Using Anti-HER2 Antibody-Conjugated Gold Nanoparticles

Hyeon-Jun JEON; Byul-Bo-Ra CHOI; Kwang-Ha PARK; Dae-Seok HWANG; Uk-Kyu KIM; Gyoo-Cheon KIM

Hyeon-Jun JEON; Byul-Bo-Ra CHOI; Kwang-Ha PARK; Dae-Seok HWANG; Uk-Kyu KIM; Gyoo-Cheon KIM.

Yonsei Medical Journal ; : 509-516, 2019.

Article in English | WPRIM | ID: wpr-762085

ABSTRACT

ABSTRACT

PURPOSE:

This study was conducted to verify the induction and mechanism of selective apoptosis in G361 melanoma cells using anti-HER2 antibody-conjugated gold nanoparticles (GNP-HER2). MATERIALS AND

METHODS:

Following GNP-HER2 treatment of G361 cells, cell cycle arrest and apoptosis were measured by WST-1 assay, Hemacolor staining, Hoechst staining, immunofluorescence staining, fluorescence-activated cell sorting analysis, and Western blotting.

Subject(s)

Actins; Apoptosis Inducing Factor; Apoptosis; Blotting, Western; Caspase 3; Caspases; Cell Adhesion; Cell Cycle; Cell Cycle Checkpoints; Cell Death; Cyclin A; Cyclin D1; Cyclin E; Cyclins; Cytochromes c; Cytoplasm; DNA Fragmentation; Down-Regulation; Flow Cytometry; Fluorescent Antibody Technique; Focal Adhesions; Melanoma; Mitochondria; Nanoparticles; Phosphotransferases; ErbB Receptors; Up-Regulation

HER2; melanoma cells; cell cycle arrest; apoptosis; conjugation

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphotransferases / Down-Regulation / Cell Adhesion / Cell Cycle / Up-Regulation / Blotting, Western / Fluorescent Antibody Technique / Actins / Cell Death / Apoptosis Language: English Journal: Yonsei Medical Journal Year: 2019 Type: Article

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