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Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Model
Yonsei Medical Journal ; : 854-863, 2019.
Article in English | WPRIM | ID: wpr-762121
ABSTRACT

PURPOSE:

Relaxin (RLX) is a transforming growth factor-β1 (TGF-β1) antagonist that is believed to function as a potent collagen re-arranger and a major suppressor of extracellular matrix components. Adenoviruses (Ads) are accepted vectors for cancer gene therapy. However, repeated treatments of Ad are limited by short-term biological activity in vivo. The efficacy of sustained RLX expression to scar remodeling was assessed using an injectable alginate gel-matrix system. MATERIALS AND

METHODS:

Pig scar tissue was treated with relaxin-expressing Ad loaded in alginate gel (gel/Ad-RLX). Surface areas, color, and pliability of scars were compared, and various factors influencing scar formation and collagen arrangement were analyzed.

RESULTS:

Gel/Ad-RLX decreased scar size, color index, and pliability. Immunohistochemistry showed decreased levels of major extracellular matrix proteins in the gel/Ad-RLX-treated group. Furthermore, treatment with gel/Ad-RLX reduced expression of tissue inhibitor of metalloproteinase-1 and alpha-smooth muscle actin and markedly increased expression of matrix metalloproteinase-1 in pig scar tissues. Gel/Ad-RLX also significantly downregulated TGF-β1 and upregulated TGF-β3 mRNAs in pig scar tissues.

CONCLUSION:

These results support a prominent role for RLX in scar remodeling and suggest that gel/Ad-RLX may have therapeutic effects on scar formation.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Relaxin / RNA, Messenger / Immunohistochemistry / Genetic Therapy / Adenoviridae / Extracellular Matrix Proteins / Actins / Collagen / Cicatrix / Pliability Type of study: Prognostic study Language: English Journal: Yonsei Medical Journal Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Relaxin / RNA, Messenger / Immunohistochemistry / Genetic Therapy / Adenoviridae / Extracellular Matrix Proteins / Actins / Collagen / Cicatrix / Pliability Type of study: Prognostic study Language: English Journal: Yonsei Medical Journal Year: 2019 Type: Article