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Respiratory syncytial virus increases eosinophil extracellular traps in a murine model of asthma
Asia Pacific Allergy ; (4): e32-2019.
Article in English | WPRIM | ID: wpr-762877
ABSTRACT

BACKGROUND:

Respiratory viral infections are the leading cause of asthma exacerbations. Eosinophil activation results in the formation of eosinophil extracellular traps (EETs), which release web-like structures of DNA and proteins that bind, disarm and extracellularly kill pathogens.

OBJECTIVE:

We investigated whether the respiratory syncytial virus (RSV) in vitro could induce EETs in bronchoalveolar lavage fluid eosinophils in a murine model of asthma.

METHODS:

BALB/cJ mice (6–8 weeks old) were sensitized with 2 subcutaneous injections of ovalbumin (20 μg) on days 0 and 7, followed by three intranasal challenges with ovalbumin (100 μg) on days 14, 15, and 16 of the protocol. The control group received Dulbecco's phosphate-buffered saline. Bronchoalveolar lavage fluid eosinophils of ovalbumin group or control group were stimulated with RSV (103 PFU/mL) in vitro for 3 hours. After that, culture supernatant was collected to perform the analyses proposed in this study.

RESULTS:

We verified an increase in extracellular DNA concentration in bronchoalveolar lavage fluid eosinophils from ovalbumin group stimulated with RSV (10³ PFU/mL) in vitro, which was confirmed by confocal microscopy. We demonstrated that most cells are negative for annexin V and propidium iodide in all groups evaluated. Also, RSV in vitro decreased interferon-ɣ in culture supernatant when compared to the ovalbumin group.

CONCLUSION:

In this study, we demonstrated for the first time that RSV in vitro induces EETs formation in eosinophils from asthmatic mice.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Propidium / Respiratory Syncytial Viruses / Asthma / In Vitro Techniques / DNA / Bronchoalveolar Lavage Fluid / Ovalbumin / Annexin A5 / Microscopy, Confocal / Eosinophil Peroxidase Limits: Animals Language: English Journal: Asia Pacific Allergy Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Propidium / Respiratory Syncytial Viruses / Asthma / In Vitro Techniques / DNA / Bronchoalveolar Lavage Fluid / Ovalbumin / Annexin A5 / Microscopy, Confocal / Eosinophil Peroxidase Limits: Animals Language: English Journal: Asia Pacific Allergy Year: 2019 Type: Article