Respiratory syncytial virus increases eosinophil extracellular traps in a murine model of asthma
Asia Pacific Allergy
;
(4): e32-2019.
Article
in English
| WPRIM
| ID: wpr-762877
ABSTRACT
BACKGROUND:
Respiratory viral infections are the leading cause of asthma exacerbations. Eosinophil activation results in the formation of eosinophil extracellular traps (EETs), which release web-like structures of DNA and proteins that bind, disarm and extracellularly kill pathogens.OBJECTIVE:
We investigated whether the respiratory syncytial virus (RSV) in vitro could induce EETs in bronchoalveolar lavage fluid eosinophils in a murine model of asthma.METHODS:
BALB/cJ mice (6–8 weeks old) were sensitized with 2 subcutaneous injections of ovalbumin (20 μg) on days 0 and 7, followed by three intranasal challenges with ovalbumin (100 μg) on days 14, 15, and 16 of the protocol. The control group received Dulbecco's phosphate-buffered saline. Bronchoalveolar lavage fluid eosinophils of ovalbumin group or control group were stimulated with RSV (103 PFU/mL) in vitro for 3 hours. After that, culture supernatant was collected to perform the analyses proposed in this study.RESULTS:
We verified an increase in extracellular DNA concentration in bronchoalveolar lavage fluid eosinophils from ovalbumin group stimulated with RSV (10³ PFU/mL) in vitro, which was confirmed by confocal microscopy. We demonstrated that most cells are negative for annexin V and propidium iodide in all groups evaluated. Also, RSV in vitro decreased interferon-ɣ in culture supernatant when compared to the ovalbumin group.CONCLUSION:
In this study, we demonstrated for the first time that RSV in vitro induces EETs formation in eosinophils from asthmatic mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Propidium
/
Respiratory Syncytial Viruses
/
Asthma
/
In Vitro Techniques
/
DNA
/
Bronchoalveolar Lavage Fluid
/
Ovalbumin
/
Annexin A5
/
Microscopy, Confocal
/
Eosinophil Peroxidase
Limits:
Animals
Language:
English
Journal:
Asia Pacific Allergy
Year:
2019
Type:
Article
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