Pro-Inflammatory Role of S1P₃ in Macrophages
Biomolecules & Therapeutics
;
: 373-380, 2019.
Article
in English
| WPRIM
| ID: wpr-763026
ABSTRACT
Sphingosine kinase 1 and its product, sphingosine 1-phosphate (S1P), as well as their receptors, have been implicated in inflammatory responses. The functions of receptors S1P₁ and S1P₂ on cell motility have been investigated. However, the function of S1P₃ has been poorly investigated. In this study, the roles of S1P₃ on inflammatory response were investigated in primary perito-neal macrophages. S1P₃ receptor was induced along with sphingosine kinase 1 by stimulation of lipopolysaccharide (LPS). LPS treatment induced inflammatory genes, such iNOS, COX-2, IL-1β, IL-6 and TNF-α. TY52156, an antagonist of S1P₃ suppressed the induction of inflammatory genes in a concentration dependent manner. Suppression of iNOS and COX-2 induction was further confirmed by western blotting and NO measurement. Suppression of IL-1β induction was also confirmed by western blotting and ELISA. Caspase 1, which is responsible for IL-1β production, was similarly induced by LPS and suppressed by TY52156. Therefore, we have shown S1P₃ induction in the inflammatory conditions and its pro-inflammatory roles. Targeting S1P₃ might be a strategy for regulating inflammatory diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Sphingosine
/
Enzyme-Linked Immunosorbent Assay
/
Cell Movement
/
Blotting, Western
/
Interleukin-6
/
Caspase 1
/
Inflammation
/
Macrophages
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2019
Type:
Article
Similar
MEDLINE
...
LILACS
LIS