A Potential Therapy Using Engineered Stem Cells Prevented Malignant Melanoma in Cellular and Xenograft Mouse Models / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 797-811, 2019.
Article
in English
| WPRIM
| ID: wpr-763116
ABSTRACT
PURPOSE:
In the present study, human neural stem cells (hNSCs) with tumor-tropic behavior were used as drug delivery vehicle to selectively target melanoma. A hNSC line (HB1.F3) was transduced into two types one expressed only the cytosine deaminase (CD) gene (HB1.F3. CD) and the other expressed both CD and human interferon-β (IFN-β) genes (HB1.F3.CD. IFN-β). MATERIALS ANDMETHODS:
This study verified the tumor-tropic migratory competence of engineered hNSCs on melanoma (A375SM) using a modified Boyden chamber assay in vitro and CM-DiI staining in vivo. The antitumor effect of HB1.F3.CD and HB1.F3.CD.IFN-β on melanoma was also confirmed using an MTT assay in vitro and xenograft mouse models.RESULTS:
A secreted form of IFN-β from the HB1.F3.CD.IFN-β cells modified the epithelial-mesenchymal transition (EMT) process and metastasis of melanoma. 5-Fluorouracil treatment also accelerated the expression of the pro-apoptotic protein BAX and decelerated the expression of the anti-apoptotic protein Bcl-xL on melanoma cell line.CONCLUSION:
Our results illustrate that engineered hNSCs prevented malignant melanoma cells from proliferating in the presence of the prodrug, and the form that secreted IFN-β intervened in the EMT process and melanoma metastasis. Hence, neural stem cell-directed enzyme/prodrug therapy is a plausible treatment for malignant melanoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
In Vitro Techniques
/
Cell Line
/
Mental Competency
/
Cytosine Deaminase
/
Neural Stem Cells
/
Epithelial-Mesenchymal Transition
/
Heterografts
/
Flucytosine
/
Fluorouracil
Limits:
Animals
/
Humans
Language:
English
Journal:
Cancer Research and Treatment
Year:
2019
Type:
Article
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