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Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility / 대한생식의학회지
Clinical and Experimental Reproductive Medicine ; : 14-21, 2019.
Article in English | WPRIM | ID: wpr-763349
ABSTRACT

OBJECTIVE:

The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay.

METHODS:

In this case-control study, which received ethical committee approval, the participants were divided into fertile and infertile groups (50 patients in each group).

RESULTS:

The alkaline comet test showed the best ability to predict male infertility, followed by the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, the sperm chromatin dispersion (SCD) test, and the sperm chromatin structure assay (SCSA), while the neutral comet test had no predictive power. For our patient population, the projected cut-off point for the DNA fragmentation index was 22.08% using the TUNEL assay, 19.90% using SCSA, 24.74% using the SCD test, 48.47% using the alkaline comet test, and 36.37% using the neutral comet test. Significant correlations were found between the results of the SCD test and those obtained using SCSA and TUNEL (r =0.70 and r =0.68, respectively; p<0.001), and a statistically significant correlation was also found between the results of SCSA and the TUNEL assay (r =0.77, p<0.001). Likewise, the results of the alkaline comet test showed significant correlations with those of the SCD, SCSA, and TUNEL tests (r =0.59, r =0.57, and r =0.72, respectively; p<0.001).

CONCLUSION:

The TUNEL assay, SCSA, SCD, and the alkaline comet test were effective for distinguishing between fertile and infertile patients, and the alkaline comet test was the best predictor of male infertility.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Semen / Spermatozoa / DNA / Chromatin / Case-Control Studies / Sensitivity and Specificity / Reactive Oxygen Species / In Situ Nick-End Labeling / DNA Nucleotidylexotransferase / DNA Fragmentation Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Limits: Humans / Male Language: English Journal: Clinical and Experimental Reproductive Medicine Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Semen / Spermatozoa / DNA / Chromatin / Case-Control Studies / Sensitivity and Specificity / Reactive Oxygen Species / In Situ Nick-End Labeling / DNA Nucleotidylexotransferase / DNA Fragmentation Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Limits: Humans / Male Language: English Journal: Clinical and Experimental Reproductive Medicine Year: 2019 Type: Article