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Increased CD68/TGFβ Co-expressing Microglia/Macrophages after Transient Middle Cerebral Artery Occlusion in Rhesus Monkeys
Experimental Neurobiology ; : 458-473, 2019.
Article in English | WPRIM | ID: wpr-763781
Responsible library: WPRO
ABSTRACT
The function of microglia/macrophages after ischemic stroke is poorly understood. This study examines the role of microglia/macrophages in the focal infarct area after transient middle cerebral artery occlusion (MCAO) in rhesus monkeys. We measured infarct volume and neurological function by magnetic resonance imaging (MRI) and non-human primate stroke scale (NHPSS), respectively, to assess temporal changes following MCAO. Activated phagocytic microglia/macrophages were examined by immunohistochemistry in post-mortem brains (n=6 MCAO, n=2 controls) at 3 and 24 hours (acute stage), 2 and 4 weeks (subacute stage), and 4, and 20 months (chronic stage) following MCAO. We found that the infarct volume progressively decreased between 1 and 4 weeks following MCAO, in parallel with the neurological recovery. Greater presence of cluster of differentiation 68 (CD68)-expressing microglia/macrophages was detected in the infarct lesion in the subacute and chronic stage, compared to the acute stage. Surprisingly, 98~99% of transforming growth factor beta (TGFβ) was found colocalized with CD68-expressing cells. CD68-expressing microglia/macrophages, rather than CD206⁺ cells, may exert anti-inflammatory effects by secreting TGFβ after the subacute stage of ischemic stroke. CD68⁺ microglia/macrophages can therefore be used as a potential therapeutic target.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Primates / Brain / Immunohistochemistry / Magnetic Resonance Imaging / Transforming Growth Factor beta / Haplorhini / Microglia / Middle Cerebral Artery / Stroke / Infarction, Middle Cerebral Artery Language: English Journal: Experimental Neurobiology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Primates / Brain / Immunohistochemistry / Magnetic Resonance Imaging / Transforming Growth Factor beta / Haplorhini / Microglia / Middle Cerebral Artery / Stroke / Infarction, Middle Cerebral Artery Language: English Journal: Experimental Neurobiology Year: 2019 Type: Article