Respiratory Syncytial Virus Fusion Protein-encoding DNA Vaccine Is Less Effective in Conferring Protection against Inflammatory Disease than a Virus-like Particle Platform
Immune Network
;
: e18-2019.
Article
in English
| WPRIM
| ID: wpr-764013
ABSTRACT
Formalin-inactivated respiratory syncytial virus (RSV) vaccination causes vaccine-enhanced disease (VED) after RSV infection. It is considered that vaccine platforms enabling endogenous synthesis of RSV immunogens would induce favorable immune responses than non-replicating subunit vaccines in avoiding VED. Here, we investigated the immunogenicity, protection, and disease in mice after vaccination with RSV fusion protein (F) encoding plasmid DNA (F-DNA) or virus-like particles presenting RSV F (F-VLP). F-DNA vaccination induced CD8 T cells and RSV neutralizing Abs, whereas F-VLP elicited higher levels of IgG2a isotype and neutralizing Abs, and germinal center B cells, contributing to protection by controlling lung viral loads after RSV challenge. However, mice that were immunized with F-DNA displayed weight loss and pulmonary histopathology, and induced F specific CD8 T cell responses and recruitment of monocytes and plasmacytoid dendritic cells into the lungs. These innate immune parameters, RSV disease, and pulmonary histopathology were lower in mice that were immunized with F-VLP after challenge. This study provides important insight into developing effective and safe RSV vaccines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Respiratory Syncytial Viruses
/
Dendritic Cells
/
DNA
/
Immunoglobulin G
/
B-Lymphocytes
/
Monocytes
/
T-Lymphocytes
/
Weight Loss
/
Vaccination
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2019
Type:
Article
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