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Characterization on Responsiveness of Excitatory Synaptic Transmissions to α1-Adrenoceptor Blockers in Substantia Gelatinosa Neurons Isolated From Lumbo-Sacral Level in Rat Spinal Cords / 대한배뇨장애요실금학회지
International Neurourology Journal ; : 13-21, 2019.
Article in English | WPRIM | ID: wpr-764103
ABSTRACT

PURPOSE:

The aim of this study was to characterize the responsiveness of miniature excitatory postsynaptic currents (mEPSCs) to α1-adrenoceptor blockers in substantia gelatinosa (SG) neurons from the spinal cord to develop an explanation for the efficacy of α1-adrenoceptor blockers in micturition dysfunction.

METHODS:

Male adult Sprague-Dawley rats were used. Blind whole-cell patch-clamp recordings were performed using SG neurons in spinal cord slices. Naftopidil (100μM), tamsulosin (100μM), or silodosin (30μM), α1-adrenoceptor blockers, was perfused. The frequency of mEPSCs was recorded in an SG neuron to which the 3 blockers were applied sequentially with wash-out periods. Individual frequencies in a pair before naftopidil and tamsulosin perfusion were plotted as baseline, and the correlation between them was confirmed by Spearman correlation coefficient; linear regression was then performed. The same procedure was performed before naftopidil and silodosin perfusion. Frequencies of pairs after naftopidil and tamsulosin perfusion and after naftopidil and silodosin perfusion were similarly analyzed. The ratios of the frequencies after treatment to before were then calculated.

RESULTS:

After the treatments, Spearman ρ and the slope were decreased to 0.682 from 0.899 at baseline and 0.469 from 1.004 at baseline, respectively, in the tamsulosin group relative to the naftopidil group. In the silodosin group, Spearman ρ and the slope were also decreased to 0.659 from 0.889 at baseline and 0.305 from 0.989 at baseline, respectively, relative to the naftopidil group. Naftopidil significantly increased the ratio of the frequency of mEPSCs compared to tamsulosin and silodosin (P=0.015 and P=0.004, respectively).

CONCLUSIONS:

There was a difference in responsiveness in the frequency of mEPSCs to α1-adrenoceptor blockers, with the response to naftopidil being the greatest among the α1-adrenoceptor blockers. These data are helpful to understand the action mechanisms of α1-adrenoceptor blockers for male lower urinary tract symptoms in clinical usage.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Perfusion / Spinal Cord / Substantia Gelatinosa / Urination / Linear Models / Rats, Sprague-Dawley / Excitatory Postsynaptic Potentials / Adrenergic alpha-1 Receptor Antagonists / Lower Urinary Tract Symptoms / Neurons Limits: Animals / Humans / Male Language: English Journal: International Neurourology Journal Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Perfusion / Spinal Cord / Substantia Gelatinosa / Urination / Linear Models / Rats, Sprague-Dawley / Excitatory Postsynaptic Potentials / Adrenergic alpha-1 Receptor Antagonists / Lower Urinary Tract Symptoms / Neurons Limits: Animals / Humans / Male Language: English Journal: International Neurourology Journal Year: 2019 Type: Article