Doxorubicin Promotes Migration and Invasion of Breast Cancer Cells through the Upregulation of the RhoA/MLC Pathway / 한국유방암학회지
Journal of Breast Cancer
;
: 185-195, 2019.
Article
in English
| WPRIM
| ID: wpr-764270
ABSTRACT
PURPOSE:
Cancer cells develop acquired resistance induced by chemotherapeutic drugs. In this study, we investigated the effects of brief treatment with cytotoxic drugs on the phenotype of breast cancer cells.METHODS:
Breast cancer cells MCF7 and BT-474 were briefly treated with paclitaxel or doxorubicin. Clonogenic, migration, and invasion assays were performed on the treated cells. Western blot analysis and RhoA activity assay were also performed.RESULTS:
Breast cancer cells when briefly treated with paclitaxel or doxorubicin showed reduced clonogenic ability. Doxorubicin, but not paclitaxel, augmented cell migration and invasion. The invasion-promoting effects of doxorubicin were lost when the two drugs were sequentially used in combination. Myosin light chain (MLC) 2 phosphorylation and RhoA activity were upregulated by doxorubicin and downregulated by paclitaxel. Pretreatment with RhoA inhibitors abolished the migration- and invasion-promoting effects of doxorubicin.CONCLUSION:
Doxorubicin activates the RhoA/MLC pathway and enhances breast cancer cell migration and invasion. Therefore, this pathway might be explored as a therapeutic target to suppress anthracycline-enhanced tumor progression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Phosphorylation
/
Breast
/
Breast Neoplasms
/
Doxorubicin
/
Up-Regulation
/
Cell Movement
/
Blotting, Western
/
Paclitaxel
/
Myosin Light Chains
Language:
English
Journal:
Journal of Breast Cancer
Year:
2019
Type:
Article
Similar
MEDLINE
...
LILACS
LIS