Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes
Journal of Cancer Prevention
;
: 123-128, 2019.
Article
in English
| WPRIM
| ID: wpr-764303
ABSTRACT
BACKGROUND:
Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes.METHODS:
Human keratinocytes were pretreated with esculetin for 30 minutes and treated with H₂O₂. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay.RESULTS:
Esculetin prevented H₂O₂-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity.CONCLUSIONS:
Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Aging
/
RNA, Messenger
/
Keratinocytes
/
Blotting, Western
/
Calcium
/
Reactive Oxygen Species
/
Transcription Factor AP-1
/
Oxidative Stress
/
Matrix Metalloproteinase 1
Limits:
Humans
Language:
English
Journal:
Journal of Cancer Prevention
Year:
2019
Type:
Article
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