Mycophenolic acid mediated mitochondrial membrane potential transition change lead to T lymphocyte apoptosis / 대한외과학회지
Journal of the Korean Surgical Society
;
: 235-241, 2011.
Article
in English
| WPRIM
| ID: wpr-76451
ABSTRACT
PURPOSE:
This study demonstrated that apoptosis induced by mycophenolic acid (MPA) is mediated by mitochondrial membrane potential transition (MPT) changes in Jurkat cells.METHODS:
Cell viability and MPT changes were measured by flow cytometry. Western blotting was performed to evaluate the expression of Bcl-2 family proteins, Bid, truncated Bid (tBid), cytochrome c, voltage dependent anion channel (VDAC), poly ADP-ribose polymerase (PARP), and protein kinase C-delta (PKC-delta). The catalytic activity of caspase-9 and -3 was also measured.RESULTS:
Cell viability was decreased in time- and dose-dependent manners. Bcl-2 protein expression was decreased, but Bax protein expression was identified. A decreased Bcl-XL /Bcl-XS ratio was also noted. The expression of tBid protein also increased in a time-dependent manner in Jurkat cells treated with MPA. While normal MPT appeared as orange fluorescence, abnormal MPT corresponded to green fluorescence. Green fluorescence increased as orange decreased in the MPA-treated cells. Significantly increased concentrations of MPA induced the release of cytosolic cytochrome c. MPA also augmented the catalytic activity of caspase-9 and caspase-3 in Jurkat cells. Our findings demonstrated that MPA-induced apoptosis is mediated by MPT changes accompanied by decreased Bcl-XL expression and the appearance of tBid protein. The release of cytosolic cytochrome c from mitochondria and increased catalytic activity of caspase-9 and caspase-3 were observed in MPA-treated Jurkat cells.CONCLUSION:
These results suggest that mitochondrial dysfunction caused by MPA induces human T lymphocyte apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Lymphocytes
/
Proteins
/
Cell Survival
/
Adenosine Diphosphate Ribose
/
Blotting, Western
/
Apoptosis
/
Jurkat Cells
/
Cytosol
/
Citrus sinensis
/
Cytochromes c
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Journal of the Korean Surgical Society
Year:
2011
Type:
Article
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