Proteomic Changes in Rat Gastrocnemius Muscle After Botulinum Toxin A Injection
Annals of Rehabilitation Medicine
;
: 157-166, 2013.
Article
in English
| WPRIM
| ID: wpr-7651
ABSTRACT
OBJECTIVE:
To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of mechanisms of BoNT-A injection on skeletal muscles using proteomic elements as biomarkers.METHODS:
BoNT-A was injected into left gastrocnemius muscles of 12 Sprague-Dawley rats (2 months of age) at a dosage of 5 units/kg body weight. For the controls same volume of normal saline was injected to right gastrocnemius muscle of each rat. Muscle samples were obtained at 4 time points (3 rats per time point) 3, 7, 14, and 56 day post-injection. To reveal the alterations in muscle protein, we performed 2-dimensional electrophoresis (2DE) and compared Botox group and normal saline group at each time point. Altered protein spots in 2DE were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS) proteomics analysis.RESULTS:
Compared with normal saline group, 46 protein spots showed changed protein expression. Twelve protein spots demonstrated increased volume and 34 protein spots demonstrated decreased volume. Among spots of decreased volume, 17 spots showed statistically significant differences. Thirty-eight identified proteins were associated with alterations in energy metabolism, muscle contractile function, transcription, translation, cell proliferation, and cellular stress response.CONCLUSION:
BoNT-A gives influences on muscle contractile function and energy metabolism directly or indirectly besides neurotoxic effects. Proteomic expression provides better understanding about the effect of BoNT-A on skeletal muscle.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Body Weight
/
Botulinum Toxins
/
Electrophoresis, Gel, Two-Dimensional
/
Proteins
/
Rats, Sprague-Dawley
/
Muscle, Skeletal
/
Botulinum Toxins, Type A
/
Proteomics
/
Cell Proliferation
/
Electrophoresis
Limits:
Animals
Language:
English
Journal:
Annals of Rehabilitation Medicine
Year:
2013
Type:
Article
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