Development of Competitive Enzyme / 대한내분비학회지
Journal of Korean Society of Endocrinology
;
: 447-454, 1996.
Article
in Korean
| WPRIM
| ID: wpr-765583
ABSTRACT
Background:
Triiodothyronine(T3) is a hormone secreted from thyroid gland which exerts a stimulating effect on metabolism. The disorder of thyroid system brings about several serious diseases like hypothymidism or hyperthyroidism. Therefore, the determination of T in blood is very important on monitoring thyroid function.Methods:
Rabbit anti-T3 antibody was generated by immunization of T-BSA as an immunogen and purified hom antisera using Affi-gel protein A kit. The titer and specificity of purified antibody were characterized. To detect T3, competitive ELISA was performed using anti-T3 antibody and T3-HRP conjugate which was synthesized by glutaraldehyde method. The sensitivity and precision assay wer~e deterrnined and compared with that of RIA.Results:
The titer of purified anti-T3 antibody was about 1100 and the optimal dilution of T3- HRP conjugate was 11000. When the standard curve was constructed by ELISA, its sensitivity was about 0.5ng/ml. The eoefficient variations of intra- and inter-assay were 4.9~9.3% and 7.5~13.8%, respectively. The results obtained by ELISA and RIA correlated well with each other(n =50, r= 0.97), The linear regression equation was y= 1.09*0.08(P<0.01).Conclusion:
We successfully developed a method for the measurement of T3 on ELISA which was based on competitive reaction between antigen(T3) and enzyme labeled antigen(T3-HRP). These results demonstrated that competitive ELISA is a convenient, fast, reproducible and aecurate method for the determinstion of T in serum and can be used as practical alternative to RIA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Staphylococcal Protein A
/
Thyroid Gland
/
Triiodothyronine
/
Enzyme-Linked Immunosorbent Assay
/
Linear Models
/
Sensitivity and Specificity
/
Immunization
/
Glutaral
/
Hyperthyroidism
/
Immune Sera
Type of study:
Diagnostic study
Language:
Korean
Journal:
Journal of Korean Society of Endocrinology
Year:
1996
Type:
Article
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