Porphyromonas gingivalis accelerates atherosclerosis through oxidation of high-density lipoprotein
Journal of Periodontal & Implant Science
;
: 60-68, 2018.
Article
in English
| WPRIM
| ID: wpr-766044
ABSTRACT
PURPOSE:
The aim of this study was to evaluate the ability of Porphyromonas gingivalis (P. gingivalis) to induce oxidation of high-density lipoprotein (HDL) and to determine whether the oxidized HDL induced by P. gingivalis exhibited altered antiatherogenic function or became proatherogenic.METHODS:
P. gingivalis and THP-1 monocytes were cultured, and the extent of HDL oxidation induced by P. gingivalis was evaluated by a thiobarbituric acid-reactive substances (TBARS) assay. To evaluate the altered antiatherogenic and proatherogenic properties of P. gingivalis-treated HDL, lipid oxidation was quantified by the TBARS assay, and tumor necrosis factor alpha (TNF-α) levels and the gelatinolytic activity of matrix metalloproteinase (MMP)-9 were also measured. After incubating macrophages with HDL and P. gingivalis, Oil Red O staining was performed to examine foam cells.RESULTS:
P. gingivalis induced HDL oxidation. The HDL treated by P. gingivalis did not reduce lipid oxidation and may have enhanced the formation of MMP-9 and TNF-α. P. gingivalis-treated macrophages exhibited more lipid aggregates than untreated macrophages.CONCLUSIONS:
P. gingivalis induced HDL oxidation, impairing the atheroprotective function of HDL and making it proatherogenic by eliciting a proinflammatory response through its interaction with monocytes/macrophages.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontitis
/
Monocytes
/
Cardiovascular Diseases
/
Cholesterol
/
Tumor Necrosis Factor-alpha
/
Thiobarbituric Acid Reactive Substances
/
Porphyromonas
/
Porphyromonas gingivalis
/
Atherosclerosis
/
Foam Cells
Language:
English
Journal:
Journal of Periodontal & Implant Science
Year:
2018
Type:
Article
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