Improvement of the osteogenic potential of ErhBMP-2-/EGCG-coated biphasic calcium phosphate bone substitute: in vitro and in vivo activity
Journal of Periodontal & Implant Science
;
: 114-126, 2019.
Article
in English
| WPRIM
| ID: wpr-766094
ABSTRACT
PURPOSE:
The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG).METHODS:
The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed.RESULTS:
The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05).CONCLUSIONS:
ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
In Vitro Techniques
/
Cell Survival
/
Calcium
/
Bone Substitutes
/
Transplants
/
Escherichia
/
Bone Morphogenetic Protein 2
/
Miners
/
Maxillary Sinus
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Journal of Periodontal & Implant Science
Year:
2019
Type:
Article
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