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Improvement of the osteogenic potential of ErhBMP-2-/EGCG-coated biphasic calcium phosphate bone substitute: in vitro and in vivo activity
Journal of Periodontal & Implant Science ; : 114-126, 2019.
Article in English | WPRIM | ID: wpr-766094
ABSTRACT

PURPOSE:

The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG).

METHODS:

The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed.

RESULTS:

The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05).

CONCLUSIONS:

ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoblasts / In Vitro Techniques / Cell Survival / Calcium / Bone Substitutes / Transplants / Escherichia / Bone Morphogenetic Protein 2 / Miners / Maxillary Sinus Type of study: Prognostic study Limits: Animals / Humans / Male Language: English Journal: Journal of Periodontal & Implant Science Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoblasts / In Vitro Techniques / Cell Survival / Calcium / Bone Substitutes / Transplants / Escherichia / Bone Morphogenetic Protein 2 / Miners / Maxillary Sinus Type of study: Prognostic study Limits: Animals / Humans / Male Language: English Journal: Journal of Periodontal & Implant Science Year: 2019 Type: Article