Increase in Anti-Gal IgM Level is Associated With Early Graft Failure in Intraportal Porcine Islet Xenotransplantation
Annals of Laboratory Medicine
;
: 611-617, 2015.
Article
in English
| WPRIM
| ID: wpr-76933
ABSTRACT
BACKGROUND:
Anti-Gal is a major antibody induced in non-human primates (NHPs) after xenotransplantation. To understand the mechanism of graft rejection, we investigated the association between anti-Gal responses and graft failure in NHP recipients of porcine islet transplantation (PITx).METHODS:
Intraportal PITx was performed in 35 diabetic NHPs, and graft function was monitored. Early graft failure (EGF) was defined as loss of graft function within a month after PITx. Seven, 19, nine NHPs received immunosuppression (IS) without CD40 pathway blockade (Group I), with anti-CD154 (Group II), and with anti-CD40 (Group III), respectively. The anti-Gal levels on day 0 and day 7 of PITx were measured by ELISA.RESULTS:
The frequency of EGF was significantly lower in Group II (26.3%) than in Group I (100%, P=0.0012) and Group III (77.8%, P=0.0166). While levels of anti-Gal IgG in Group I and anti-Gal IgM in Group III increased on day 7 compared with day 0 (P=0.0156 and 0.0273), there was no increase in either on day 7 in Group II. The ratio of anti-Gal IgM or IgG level on day 7 to that on day 0 (Ratio7/0) was significantly higher in recipients with EGF than without EGF (P=0.0009 and 0.0027). ROC curve analysis of anti-Gal IgM Ratio7/0 revealed an area under the curve of 0.789 (P=0.0003).CONCLUSIONS:
IS with anti-CD154 suppressed anti-Gal responses and prevented EGF in PITx. Anti-Gal IgM Ratio7/0, being associated with EGF, is a predictive marker for EGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Transplantation, Heterologous
/
Immunoglobulin G
/
Immunoglobulin M
/
ROC Curve
/
Islets of Langerhans Transplantation
/
CD40 Antigens
/
Area Under Curve
/
CD40 Ligand
/
Disaccharides
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS