Nur77 upregulates HIF-alpha by inhibiting pVHL-mediated degradation
Experimental & Molecular Medicine
;
: 71-83, 2008.
Article
in English
| WPRIM
| ID: wpr-77112
ABSTRACT
In this study, we investigated the role of Nur77, an orphan nuclear receptor, in HIF-alpha transcriptional activity. We found that Nur77 associates and stabilizes HIF-1alpha via indirect interaction. Nur77 was found to interact with pVHL in vivo via the alpha-domain of pVHL. By binding to pVHL, Nur77 competed with elongin C for pVHL binding. Moreover, Nur77-binding to pVHL inhibited the pVHL-mediated ubiquitination of HIF-1alpha and ultimately increased the stability and transcriptional activity of HIF-1alpha. The ligand-binding domain of Nur77 was found to interact with pVHL and the expression of this ligand-binding domain was sufficient to stabilize and transactivate HIF-1alpha. Under the conditions that cobalt chloride was treated or pVHL was knocked down, Nur77 could not stabilize HIF-alpha. Moreover, Nur77 could not further stabilize HIF-2alpha in A498/VHL stable cells, which is consistent with our finding that Nur77 indirectly stabilizes HIF-alpha by binding to pVHL. Thus, our results suggest that an orphan nuclear receptor Nur77 binds to pVHL, thereby stabilizes and increases HIF-alpha transcriptional activity under the non- hypoxic conditions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Thermodynamics
/
Transcription Factors
/
Receptors, Steroid
/
Transcriptional Activation
/
Up-Regulation
/
Protein Processing, Post-Translational
/
PC12 Cells
/
Protein Structure, Tertiary
/
Receptors, Cytoplasmic and Nuclear
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2008
Type:
Article
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