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Application and optimization of CRISPR/Cas system in bacteria / 生物工程学报
Chinese Journal of Biotechnology ; (12): 341-350, 2019.
Article in Chinese | WPRIM | ID: wpr-771372
ABSTRACT
Clustered regular interspaced short palindromic repeats (CRISPR) system has been widely used in recent years. Compared with traditional genome editing technology, CRISPR/Cas system has notable advantages, including high editing efficiency, high specificity, low cost and the convenience for manipulation. Type Ⅱ and Ⅴ CRISPR/Cas system only requires a single Cas9 protein or a single Cpf1 protein as effector nucleases for cutting double-stranded DNA, developed as genome editing tools. At present, CRISPR/Cas9 technology has been successfully applied to the genome editing of eukaryotes such as zebrafish, mice and human cells, whereas limited progress has been made in the genome editing of bacteria. In our review, we describe CRISPR/Cas system, its mechanism and summarize the optimization and progress of genome editing in bacteria.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacteria / Endonucleases / CRISPR-Cas Systems / Gene Editing Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacteria / Endonucleases / CRISPR-Cas Systems / Gene Editing Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2019 Type: Article