Methods for mammalian single cell research - a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 27-39, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-771403
ABSTRACT
Basic research in life science and medicine has dug into single cell level in recent years. Single-cell analysis offers to understand life from diverse perspectives and is used to profile cell heterogeneity to investigate mechanism of diseases. Single cell technologies have also found applications in forensic medicine and clinical reproductive medicine, while the techniques are rapidly evolving and have become more and more sophisticated. In this article, we reviewed various single cell isolation techniques and their pros and cons, including manual cell picking, laser capture microdissection and microfluidics, as well as analysis methods for DNA, RNA and protein in single cell. In addition, we summarized major up-to-date single cell research achievements and their potential applications.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
RNA
/
Cell Separation
/
Single-Cell Analysis
/
Laser Capture Microdissection
Type of study:
Practice guideline
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2019
Type:
Article
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