IFN-γ induces overexpression of PD-L1 and epithelialmesenchymal transformation of breast cancer cells through activating ERK/Jak2-STAT signaling pathways / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2007-2015, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-771408
ABSTRACT
To investigate the effect of INF-γ on the expression of programmed death ligand 1 (PD-L1), epithelial-mesenchymal transition (EMT) and the potential mechanism in breast cancer cell line MDA-MB-231, the cells were treated with different concentrations of INF-γ. The expressions of proteins, including PD-L1, cell-migration-related proteins (E-cadherin, N-cadherin and vimentin), ERK, p-ERK, Jak2, and p-Jak2 were detected by Western blotting analysis and immunofluorescent staining assay. Cell migration was studied through cell wound healing assay and transwell assay. IFN-γ could up-regulate the expressions of PD-L1 in MDA-MB-231 cells. The cell migration rate was significantly increased after adding IFN-γ. The expression levels of vimentin and N-cadherin were increased whereas the expression of E-cadherin was decreased after adding IFN-γ. The expression levels of ERK, p-ERK, Jak2 and p-Jak2 were significantly increased and this phenomenon was inhibited when adding ERK inhibitor U0126 or Jak2 inhibitor AG490. These results demonstrate that IFN-γ could up-regulate the expression of PD-L1, promote cell migration and transmission, and facilitate epithelial-mesenchymal transformation of breast cancer cells and this process may be related with ERK and Jak2-STAT signaling pathways.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Breast Neoplasms
/
Signal Transduction
/
Cell Movement
/
Interferon-gamma
/
Cell Line, Tumor
/
Extracellular Signal-Regulated MAP Kinases
/
STAT Transcription Factors
/
Janus Kinase 2
/
Epithelial-Mesenchymal Transition
/
B7-H1 Antigen
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2018
Type:
Article
Similar
MEDLINE
...
LILACS
LIS