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Mechanism of β-carboline alkaloids inhibiting migration and invasion of SGC-7901 cells / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 119-124, 2019.
Article in Chinese | WPRIM | ID: wpr-771508
ABSTRACT
To explore the mechanism of β-carboline alkaloids inhibiting the migration and invasion of SGC-7901 cells and its correlation with FAK gene expression,CCK-8 method was used to determine the inhibitory rate of β-carboline alkaloids on the proliferation of gastric cancer SGC-7901 cells under different concentrations.The effect of β-carboline alkaloids on the migration and invasion of SGC-7901 cells was used by Transwell compartment.Detection of mRNA and protein expression of FAK genes were used by qRT-PCR and Western blot.Then si-FAK-1051 recombinant plasmid was transfected into SGC-7901 cells.FAK gene silencing effect was identified by qRT-PCR and Western blot technique again.Finally,the effects of FAK gene silencing on proliferation and migration of gastric cancer SGC-7901 cells were detected by CCK-8 kit and Transwell chamber assay respectively.With the increase of the concentration ofβ-carboline alkaloids,the inhibitory rate of SGC-7901 cells in human gastric cancer cells increased gradually,with IC5013.364 mg·L-1.The number of SGC-7901 cells of Transwell compartment in the positive experimental group(5-FU,5 mg·L-1) and the β-carboline alkaloids group decreased significantly(P<0.01) and the number of SGC-7901 cells in the β-carboline alkaloids group was significantly lower than that in the positive experimental group(P<0.01).Compared with the blank control group,the mRNA and protein expression level of FAK genes in the positive experimental group was significantly lower than that in the experimental group of β-carboline alkaloids(P<0.05).After transfection of si-FAK-1051 into gastric cancer SGC-7901 cells,the expression of mRNA and protein of FAK gene was significantly down regulated(P<0.05).SGC-7901 cell proliferation and cell migration ability also decreased significantly(P<0.05).β-carboline alkaloids are more effective than 5-FU in inhibiting migration and invasion of gastric cancer SGC-7901 cells,and the mechanism may be related to the inhibition of mRNA and protein expression of FAK gene by β-carboline alkaloids.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Stomach Neoplasms / Carbolines / Gene Expression Regulation, Neoplastic / Cell Movement / Gene Silencing / Cell Line, Tumor / Cell Proliferation / Drug Therapy Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Stomach Neoplasms / Carbolines / Gene Expression Regulation, Neoplastic / Cell Movement / Gene Silencing / Cell Line, Tumor / Cell Proliferation / Drug Therapy Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article