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Application of DNA metabarcoding in species identification of Chinese herbal medicines / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1-8, 2019.
Article in Chinese | WPRIM | ID: wpr-771525
ABSTRACT
DNA metabarcoding,one rapid and robust method using specific standard DNA fragments,has been widely used for rapid species identification of a bulk sample through high-throughput sequencing technologies.While it has been widely used in the studies of metagenomics,animal and plant biodiversity,it has gradually come to be used as a profitable method in species identification of mixed Chinese herbal medicines.In this paper,we mainly summarize the current studies of the application of DNA metabarcoding in species identification of mixed Chinese herbal medicines.Moreover,high-throughput sequencing technologies adopted in those studies,such as Sanger,the next-generation,and third-generation sequencing technologies,are discussed.It is conducted to provide a theoretical guidance for the application of DNA metabarcoding in species identification of mixed Chinese herbal medicines and in more other biodiversity studies.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Drugs, Chinese Herbal / Classification / DNA, Plant / Biodiversity / DNA Barcoding, Taxonomic / High-Throughput Nucleotide Sequencing / Genetics Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Drugs, Chinese Herbal / Classification / DNA, Plant / Biodiversity / DNA Barcoding, Taxonomic / High-Throughput Nucleotide Sequencing / Genetics Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article