A real-time fluorescence quantitative PCR method for source identification of Crocus sativus and Carthamus tinctorius / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4575-4581, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-771547
ABSTRACT
The specific PCR primer was designed base on ITS2 sequence in GenBank, and we developed a SYBRGreen real-time fluorescence quantitative PCR system for identification of Crocus sativus and Carthamus tinctorius source. Compared with Chinese herbal medicine DNA barcode technique, this method showed characteristics of shorter time, higher specificity and sensitivity. Using this method to detect 15 samples, 4 were C. sativus, 8 were C. tinctorius, and the other 3 samples were none of them. The result was in accordance with Chinese herbal medicine DNA barcode. This study lays the foundation for identification of related Chinese medical materials.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Crocus
/
Carthamus tinctorius
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2018
Type:
Article
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