Construction of yeast two-hybrid library of Salvia miltiorrhiza and screening of SmJAZ8 interaction protein / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 484-492, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-771711
ABSTRACT
The study is aimed to construct high quality Salvia miltiorrhiza cDNA library and obtain the SmJAZ8 gene of S. miltiorrhiza by yeast two-hybrid system. In this study, full-length cDNA was synthesized from roots, stems, leaves, flowers and hairy roots of S. miltiorrhiza. The full-length cDNA library was synthesized by SMART method and constructed with DSN homogenization technique. The results showed that the library capacity was 1.45×10⁶, the recombination rate was 100%, and the average size of the insert was 500-2 000 bp. The recombinant vector of pDEST-pGADT7-SmJAZ8 was constructed and transformed into Y2HGold strain. The interaction protein was screened by yeast two-hybrid system. The DnaJ protein and UBQ protein were screened by yeast two-hybrid system. This study has successfully constructed a full-length cDNA library of S. miltiorrhiza, and laid the foundation for the follow-up study on functional gene screening and gene function of S. miltiorrhiza.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Proteins
/
Gene Library
/
DNA, Complementary
/
Two-Hybrid System Techniques
/
Salvia miltiorrhiza
/
Co-Repressor Proteins
/
Genetics
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Screening study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2018
Type:
Article
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