Biosynthesis of α-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1266-1276, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-771802
ABSTRACT
In a one-step fermentation system of vitamin C production with Gluconobacter oxydans and Ketogulonicigenium vulgare, a functional module of α-lipoic acid biosynthesis was constructed in G. oxydans. The engineered G. oxydans was co-cultured with K. vulgare to enhance the growth and 2-keto-L-gulonic acid (2-KGA) production of K. vulgare. This one-step fermentation system alleviated the growth inhibition during the mono-culture of K. vulgare and strengthened the interaction between the two bacteria. Moreover, the yield of vitamin C precursor (2-KGA) increased to 73.34 g/L (the control group was 59.09 g/L), and the conversion of D-sorbitol to 2-KGA increased to 86.0%. This study provides a new idea for further optimizing the one-step fermentation system of vitamin C production.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ascorbic Acid
/
Thioctic Acid
/
Gluconobacter oxydans
/
Rhodobacteraceae
/
Fermentation
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2019
Type:
Article
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