Imprinting genes modified parthenogenetic embryonic stem cells produce full-term mouse via tetraploid complementation / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 910-918, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-771835
ABSTRACT
Parthenogenetic embryonic stem cells (pESCs) derived from bi-maternal genomes do not have competency of tetraploid complementation, due to lacking of paternal imprinting genes. To make pESCs possess fully development potentials and similar pluripotency to zygote-derived ESCs, we knocked out one allelic gene of the two essential maternal imprinting genes (H19 and IG) in their differentially methylated regions (DMR) via CRISPR/Cas9 system and obtained double knock out (DKO) pESCs. Maternal pESCs had similar morphology, expression levels of pluripotent makers and in vitro neural differentiation potentials to zygotes-derived ESCs. Besides that, DKO pESCs could contribute to full-term fetuses through tetraploid complementation, proving that they held fully development potentials. Derivation of DKO pESCs provided a type of major histocompatibility complex (MHC) matched pluripotent stem cells, which would benefit research in regenerative medicine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parthenogenesis
/
Genomic Imprinting
/
Pluripotent Stem Cells
/
Regenerative Medicine
/
Embryonic Stem Cells
/
Gene Knockout Techniques
/
Tetraploidy
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2019
Type:
Article
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