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Oxidative Damage of Bone Marrow Stromal Cells Caused by Chemotherapy Drugs / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 970-975, 2019.
Article in Chinese | WPRIM | ID: wpr-771853
ABSTRACT
OBJECTIVE@#To explore the oxidative damage of OP9 cells induced by daunorubicin (DNR) treatment.@*METHODS@#The TMRM probe was used to detect mitochondrial membrane potential by flow cytometry; the reactive oxygen species (ROS) was determined by flow cytometry DCFDA probe; the real-time PCR was used to detect the molecular expression of antioxidant enzymeglutathione peroxidase (GPX) in OP9 cells; the expression of γ-H2AX was determined by flow cytometry.@*RESULTS@#Compared with normal OP9 cells, the positive rate of TMRM in DNR-treated OP9 cells decreased by 56.7% (P<0.05); the positive rate of DCFDA in DNR-treated OP9 cells increased by 3.52 times (P<0.01). Compared with normal OP9 cells, DNR-treated OP9 cells showed a decrease in the expression of GPX4 by 44.22% (P<0.001); the expression of GPX7 decreased by 65.7% (P<0.001); the expression of GPX8 decreased by 24.7% (P<0.001); the positive rate of γ-H2AX in DNR-treated OP9 cells increased (P<0.05).@*CONCLUSION@#After DNR treatment, mitochondrial membrane potential of OP9 cells decreases; the level of reactive oxygen species increases; the expression of glutathione peroxidase (GPX) molecules decreases significantly; genomic instability increases obviously; the oxidative damage of cells increased.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Daunorubicin / Reactive Oxygen Species / Apoptosis / Oxidative Stress / Mesenchymal Stem Cells Language: Chinese Journal: Journal of Experimental Hematology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Daunorubicin / Reactive Oxygen Species / Apoptosis / Oxidative Stress / Mesenchymal Stem Cells Language: Chinese Journal: Journal of Experimental Hematology Year: 2019 Type: Article