Increment of N-cadherin Expression in Bone Marrow Mesenchymal Stem Cells Accompanies by Imparment of Bone Marrow B Lymphocyte Production in MRL/lpr Lupus Mice / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 964-969, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-771854
ABSTRACT
OBJECTIVE@#To investigate the hematological changes in MRL/lpr lupus mice and detect N-cadherin expression in their bone marrow mesenchymal stem cell (BMMSC).@*METHODS@#Peripheral blood cell count was analyzed. The ratio of each lineage in bone marrow was analyzed by flow cytometry. Bone marrow CFU-pre-B, BFU-E and CFU-GM were detected by colony formation assay. Expression of N-cadherin in BMMSC was analyzed by Western blot before and after treatment with the BMP/Smad pathway aganist BMP-2 and inhibitor Noggin.@*RESULTS@#Hemoglobin, red blood cell count and hematocrit decreased in lupus mice, compared with C57BL/6 mice. The ratio of B220+ lymphocyte and the number of CFU-pre-B in bone marrow of lupus mice decreased. Expression of N-cadherin in BMMSC of lupus mice was higher than that in the control group. Expression of N-cadherin decreased in BMP-2-treated BMMSC and increased after Noggin treatment.@*CONCLUSION@#Hematological changes in lupus mice include anemia and impairment of bone marrow B cell production. The expression of N-cadherin in BMMSC of lupus mice increases which maybe involved in abnormal hemogenesis in MRI/Ipr lupus mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow
/
Bone Marrow Cells
/
B-Lymphocytes
/
Cadherins
/
Mice, Inbred MRL lpr
/
Mesenchymal Stem Cells
/
Mice, Inbred C57BL
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2019
Type:
Article
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