Mutation analysis in a large Chinese pedigree affected with preaxial polydactyly II / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 610-612, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-771956
ABSTRACT
OBJECTIVE@#To detect potential mutation in a large pedigree affected with preaxial polydactyly II.@*METHODS@#With informed consent obtained, peripheral blood samples were collected from the proband, her family members as well as 100 healthy controls. Genomic DNA was extracted. The zone of polarizing activity regulatory sequence (ZRS) of the SHH gene was amplified by PCR and subjected to bi-directional Sanger sequencing.@*RESULTS@#The pedigree had typical preaxial polydactyly II. A heterozygous C>G mutation at position 105 of the ZRS region was detected in all patients but none of the unaffected members and 100 healthy controls.@*CONCLUSION@#The heterozygous 105C>G mutation of the ZRS region probably underlies the disease in this pedigree.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pedigree
/
Thumb
/
DNA Mutational Analysis
/
Polydactyly
/
Mutation
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2019
Type:
Article
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