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Association of EGFR gene G719S and T790M mutations with cervical cancer / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 376-379, 2019.
Article in Chinese | WPRIM | ID: wpr-772003
ABSTRACT
OBJECTIVE@#To establish a rapid and accurate "on/off" switch technique consisted of 3'-phosphorothioate-modified allele-specific primers and exo+ polymerase to screen the G719S and T790M mutations of epidermal growth factor receptor (EGFR) gene. The switch was used to identify cervical cancer patients who are sensitive to tyrosine kinase inhibitor (TKI).@*METHODS@#Allele-specific primers targeting recombinant wild-type and mutation-type templates were designed with 3' terminal phosphorothioate modification. Two-directional primer extension was carried out using Pfu polymerase. The G719S and T790M mutations were detected by the technique among cervical cancer tissues. The results were verified by Sanger sequencing.@*RESULTS@#No mutation was detected among the 80 cervical cancer cases, and the results were consistent with that of Sanger sequencing. No significant difference was found between the frequencies of the G719S and T790M mutations between the patient and the control groups (P> 0.05).@*CONCLUSION@#A sensitive "on/off" switch technique for detecting the two EGFR mutations was established. The G719S and T790M mutations are not associated with cervical cancer.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Uterine Cervical Neoplasms / Carcinoma, Non-Small-Cell Lung / Genes, erbB-1 / Drug Resistance, Neoplasm / Protein Kinase Inhibitors / ErbB Receptors / Genetics / Lung Neoplasms / Mutation Type of study: Prognostic study Limits: Female / Humans Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Uterine Cervical Neoplasms / Carcinoma, Non-Small-Cell Lung / Genes, erbB-1 / Drug Resistance, Neoplasm / Protein Kinase Inhibitors / ErbB Receptors / Genetics / Lung Neoplasms / Mutation Type of study: Prognostic study Limits: Female / Humans Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2019 Type: Article