TLR/NF-κB independent signaling pathway in TNF-α suppression of diabetic MyD88-knockout mice after polysaccharides administration / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 35-40, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-772601
ABSTRACT
OBJECTIVE@# To investigate the effect of polysaccharides (LBPs) on TLR/NF-κB independent pathway and serum tumor necrosis factor (TNF-α) level in diabetic MyD88-knockout mice.@*METHODS@# Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit.@*RESULTS@# Real time RT-PCR showed that compared with model group, the relative expressions of and mRNA in macrophages of LBPs group were significantly decreased and expression of was significantly increased (all 0.05).@*CONCLUSIONS@# LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Blood
/
Drugs, Chinese Herbal
/
Signal Transduction
/
Random Allocation
/
Gene Expression Regulation
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Mice, Knockout
/
Macrophages, Peritoneal
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2018
Type:
Article
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