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Astragaloside Ⅳ regulates Nrf2/Bach1/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2331-2337, 2019.
Article in Chinese | WPRIM | ID: wpr-773090
ABSTRACT
Astragaloside Ⅳ(AS-Ⅳ) has protective effects against ischemia-reperfusion injury(IRI), but its mechanism of action has not yet been determined. This study aims to investigate the protective effects and mechanism of AS-Ⅳ on H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation(H/R). The H/R model of myocardial cells was established by hypoxic culture for 12 hours and then reoxygenation culture for 8 hours. After AS-Ⅳ treatment, cell viability, the reactive oxygen species(ROS) levels, as well as the content or activity of superoxide dismutase(SOD), malondialdehyde(MDA), interleukin 6(IL-6), and tumor necrosis factor alpha(TNF-α), were measured to evaluate the effect of AS-Ⅳ treatment. The effect of AS-Ⅳ on HO-1 protein expression and nuclear Nrf2 and Bach1 protein expression was determined by Western blot. Finally, siRNA was used to knock down HO-1 gene expression to observe its reversal effect on AS-Ⅳ intervention. The results showed that as compared with the H/R model group, the cell viability was significantly increased(P<0.01), ROS level in the cells, MDA, hs-CRP and TNF-α in cell supernatant and nuclear protein Bach1 expression in the cells were significantly decreased(P<0.01), while SOD content, HO-1 protein expression in cells and expression of nuclear protein Nrf2 were significantly increased(P<0.01) in H/R+AS-Ⅳ group. However, pre-transfection of HO-1 siRNA into H9c2 cells by liposome could partly reverse the above effects of AS-Ⅳ after knocking down the expression of HO-1. This study suggests that AS-Ⅳ has significant protective effect on H/R injury of H9c2 cardiomyocytes, and Nrf2/Bach1/HO-1 signaling pathway may be a key signaling pathway for the effect.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Saponins / Triterpenes / Signal Transduction / Cell Hypoxia / Cells, Cultured / Apoptosis / Myocytes, Cardiac / Heme Oxygenase-1 / Basic-Leucine Zipper Transcription Factors Type of study: Prognostic study Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Saponins / Triterpenes / Signal Transduction / Cell Hypoxia / Cells, Cultured / Apoptosis / Myocytes, Cardiac / Heme Oxygenase-1 / Basic-Leucine Zipper Transcription Factors Type of study: Prognostic study Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article