Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2046-2050, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-773130
ABSTRACT
The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyrans
/
DNA, Fungal
/
China
/
Classification
/
DNA, Ribosomal Spacer
/
Scrophularia
/
Iridoid Glycosides
/
Endophytes
/
Fungi
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2019
Type:
Article
Similar
MEDLINE
...
LILACS
LIS