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Rapid identification of Cordyceps and its products using DNA barcoding method / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1965-1973, 2019.
Article in Chinese | WPRIM | ID: wpr-773141
ABSTRACT
Cordyceps is one of the most valuable traditional Chinese medicines. There are various counterfeits in markets because of high price and limited output. In this study,116 Cordyceps,146 hosts and 29 related products were collected and detected by using normal DNA barcoding technology and specific PCR method. The results indicated that Cordyceps and its adulterants could be distinguished from each other through DNA barcoding technology based on ITS and COⅠsequences. Two pairs specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 were developed to amplify 297 bp and 136 bp ITS regions of Cordyceps sinensis,respectively. It could be used to identify C. sinensis specifically and rapidly. Furthermore,specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 combined with ITS and COⅠsequences could differentiate powder Cordyceps from fermentation mycelia and could identify related products. Therefore,the method developed from this study could be applied to identify the powder of Cordyceps from fermentation mycelia and related products efficiently.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymerase Chain Reaction / Classification / DNA Primers / Mycelium / Cordyceps / DNA Barcoding, Taxonomic Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymerase Chain Reaction / Classification / DNA Primers / Mycelium / Cordyceps / DNA Barcoding, Taxonomic Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article