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Inhibitory effect of connexin43 protein on autophagy in cisplatin-resistant testicular cancer I-10 cells / 南方医科大学学报
Article in Zh | WPRIM | ID: wpr-773488
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of connexin43 (Cx43) protein on autophagy in cisplatin (DDP)-resistant testicular cancer I-10 cells.@*METHODS@#The expression of Cx43 proteins in testicular cancer I-10 cells and I-10/DDP cells were detected with Western blotting. I-10/DDP cells were transfected with a full- length mouse Cx43 vector (mCx43) Lipofectamine, the empty vector or Lipofectamine (blank control group), and the changes in the expressions of LC3 and p62 proteins were determined with Western blotting. mCherry-GFP-LC3B transfection and transmission electron microscopy were used to analyze the changes in autophagy of the cells with Cx43 overexpression.@*RESULTS@#Cx43 was significantly decreased in I-10/DDP cells compared with I-10 cells ( < 0.01). Transfection of the I-10/DDP cells with mCx43 vector resulted in significantly increased Cx43 expression in the cells ( < 0.01) and caused significantly decreased expression of LC3-Ⅱ ( < 0.01) and increased expression of p62 ( < 0.05) as compared with the negative control cells. Both transmission electron microscopy and mCherry-GFP-LC3B transfection showed that the number of autophagosomes was obviously reduced in mCx43-transfected cells as compared with the negative control cells.@*CONCLUSIONS@#Cx43 inhibits autophagy in cisplatin-resistant testicular cancer I-10 /DDP cells.
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Full text: 1 Index: WPRIM Main subject: Pathology / Autophagy / Testicular Neoplasms / Cisplatin / Connexin 43 / Drug Resistance, Neoplasm / Cell Line, Tumor / Metabolism Limits: Animals Language: Zh Journal: Journal of Southern Medical University Year: 2019 Type: Article
Full text: 1 Index: WPRIM Main subject: Pathology / Autophagy / Testicular Neoplasms / Cisplatin / Connexin 43 / Drug Resistance, Neoplasm / Cell Line, Tumor / Metabolism Limits: Animals Language: Zh Journal: Journal of Southern Medical University Year: 2019 Type: Article