Inhibiting miR-186 expression alleviates mitochondrial damage in hypoxic human umbilical vein endothelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 898-903, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-773515
ABSTRACT
OBJECTIVE@#To investigate the effect of miR-186 inhibition on the expression of hypoxia-inducible factor-1α (HIF-α) and mitochondrial function in hypoxic vascular endothelial cells.@*METHODS@#Human umbilical vein endothelial cells (HUVECs) cultured in routine or hypoxic conditions for 6 h were examined for the expression of miR-186. A miR-186 inhibitor was transfected in the HUVECs, and the cells were subsequently cultured in hypoxic condition for 6 h to observe the changes in the mitochondrial structure under an electron microscope. The changes in the mRNA and protein expressions of HIF-1α in response to miR-186 interference were tested using real-time fluorescent quantitative PCR and Western blotting.@*RESULTS@#The expression of miR-18 was mildly increased in HUVECs after hypoxic exposure for 6 h (=0.0188). Interference of miR-186 expression obviously promoted the mRNA and protein expressions of HIF-1α in HUVECs. In hypoxic conditions, miR-186 interference significantly reduced mitochondrial damage in HUVECs as observed under electron microscope (=0.0297).@*CONCLUSIONS@#Inhibition of miR-186 protects vascular endothelial cells against hypoxic injuries by promoting HIF-α expression to lessen mitochondrial damage, suggesting the possibility of targeted miR-186 interference for treatment of hemorrhagic shock.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Umbilical Veins
/
Cell Hypoxia
/
MicroRNAs
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Human Umbilical Vein Endothelial Cells
/
Hypoxia
/
Mitochondria
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2019
Type:
Article
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