Design, screening and antimicrobial activity of novel peptides against / 南方医科大学学报
Journal of Southern Medical University
;
(12): 823-829, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-773526
ABSTRACT
OBJECTIVE@#To construct antimicrobial peptides with potent antimicrobial activity, low cytotoxicity and efficient killing rate of for prevention and treatment of dental caries.@*METHODS@#We exploited the existing design strategies to modify reutericin 6 or gassericin A produced by species in the oral cavity based on their cationicity, amphipathicity and -helical structure. We examined their antimicrobial activities using bacterial susceptibility assay, their cytotoxicity through cytotoxicity assay and their killing rate of with time-kill assay. We further evaluated the candidate derivatives for their killing rate against , their antimicrobial activity against different oral pathogens and the development of drug resistance.@*RESULTS@#We constructed 6 AT-1 derivatives, among which AT-7 showed an MIC of 3.3 μmol/L against , and with a killing rate of 88.7% against within 5 min. We did not obtain strains of resistant to AT- 7 after induction for 10 passages.@*CONCLUSIONS@#Hydrophobicity and imperfect amphipathic structure are two key parameters that define the antimicrobial potency of the antimicrobial peptides. The imperfectly amphipathic peptide AT-7 shows the potential for clinical application in dental caries treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Streptococcus mutans
/
Microbial Sensitivity Tests
/
Dental Caries
/
Anti-Infective Agents
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2019
Type:
Article
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