A landscape of transcriptome analysis of three sclerotia growth stages in Polyporus umbellatus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3718-3723, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-773661
ABSTRACT
Polyporus umbellatus,a traditional Chinese precious medicine as long been used for eliminating dampness,diuresis and have effect on cancer,getting more and more popularly in China recently. And the developmental metabolic process of the medicinal fungus,P. umbellatus,has been gotten more attention. This study is for the first time to explore the three sclerotial growth stages in P. umbellatus,named " white Polyporus"( initial phase), " grey Polyporus"( developmental phase) and " black Polyporus"( mature phase),by utilizing the de novo transcriptome assembly analysis technology. Finally,we obtained 88. 12 Gb sequence containing85 235 unigenes( ≥200 bp) assembled and 100% were annotated. We identified genes differentially expressed among the three stages of the sclerotia and screened out MFSgst,ERG4/ERG24,WD40,Rho A,CYP450,PKS,GSase and CHS1,which may contribute to the production of medicinal secondary metabolites and the defense mechanism against the environmental stress and biological invasion. We did the qRT-PCR trial to verify our results,which is in line with expectations. Our results are purposed to unearth the molecular mechanism of the accumulation of active constituents in different stages of Polyporus sclerotia which can be applied in the production and protection of Polyporus effectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
China
/
Gene Expression Profiling
/
Polyporus
/
Transcriptome
/
Genes, Fungal
/
Genetics
/
Medicine, Chinese Traditional
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2019
Type:
Article
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