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Analysis of genomic SSRs in Pueraria lobata and P. thomsonii and establishment of DNA identity card for different germplasms of P. thomsonii of Jiangxi province / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3615-3621, 2019.
Article in Chinese | WPRIM | ID: wpr-773674
ABSTRACT
The type and frequency of simple sequence repeats( SSRs) in the genomes was investigated using the DNA sequence data of Pueraria lobata and P. thomsonii. Based on these SSRs,20 pairs of SSR primers were designed and 5 high polymorphism primer pairs were selected to analyze genetic diversity of 9 cultivars of P. thomsonii in Jiangxi province. The results showed that the 5 pairs of primers could generate 16 polymorphic alleles bands. The average polymorphism information content( PIC) of each SSR primer pair was 0. 600 7.According to the genetic similarity coefficients,the 9 cultivars of P. thomsonii can be classified into 6 germplasms. This study established DNA identity cards with 5 pairs of SSR primers for different germplasm resources of P. thomsonii in Jiangxi province,which provided reference information for the selection of fine germplasms of P. thomsonii and the theoretical basis for the study of Dao-di herbs.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymorphism, Genetic / China / DNA, Plant / Microsatellite Repeats / Genomics / Pueraria / Genetics Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymorphism, Genetic / China / DNA, Plant / Microsatellite Repeats / Genomics / Pueraria / Genetics Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2019 Type: Article