Viriditoxin Induces G2/M Cell Cycle Arrest and Apoptosis in A549 Human Lung Cancer Cells
Natural Product Sciences
;
: 282-288, 2015.
Article
in English
| WPRIM
| ID: wpr-7745
ABSTRACT
Viriditoxin is a fungal metabolite isolated from Paecilomyces variotii, which was derived from the giant jellyfish Nemopilema nomurai. Viriditoxin was reported to inhibit polymerization of FtsZ, which is a key protein for bacterial cell division and a structural homologue of eukaryotic tubulin. Both tubulin and FtsZ contain a GTP-binding domain, have GTPase activity, assemble into protofilaments, two-dimensional sheets, and protofilament rings, and share substantial structural identities. Accordingly, we hypothesized that viriditoxin may inhibit eukaryotic cell division by inhibiting tubulin polymerization as in the case of bacterial FtsZ inhibition. Docking simulation of viriditoxin to beta-tubulin indicated that it binds to the paclitaxel-binding domain and makes hydrogen bonds with Thr276 and Gly370 in the same manner as paclitaxel. Viriditoxin suppressed growth of A549 human lung cancer cells, and inhibited cell division with G2/M cell cycle arrest, leading to apoptotic cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymers
/
Tubulin
/
Paecilomyces
/
Cell Cycle
/
Cell Division
/
Cell Death
/
Paclitaxel
/
Apoptosis
/
Eukaryotic Cells
/
Polymerization
Limits:
Humans
Language:
English
Journal:
Natural Product Sciences
Year:
2015
Type:
Article
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