Glucosides of chaenomeles speciosa attenuate ischemia/reperfusion-induced brain injury by regulating NF-κB P65/TNF-α in mouse model / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 289-295, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-775221
ABSTRACT
OBJECTIVE@#To investigate the effect and mechanism of glucosides of chaenomeles speciosa (GCS) on ischemia/reperfusion-induced brain injury in mouse model.@*METHODS@#Fifty 8-week C57BL/C mice were randomly divided into five groups with 10 in each groupsham group, model group, GCS 30 mg/kg group, GCS 60 mg/kg group and GCS 90 mg/kg group, and the GCS was administrated by gavage (once a day) for 14 d. HE staining was performed to investigate the cell morphology; the Zea-Longa scores were measured for neurological activity; TUNEL staining was performed to investigate the cell apoptosis; ELISA was used to detected the oxidative stress and inflammation; Western Blot was performed to investigate the key pathway and neurological functional molecules.@*RESULTS@#Compared with the sham group, the brain tissues in model group were seriously damaged, presenting severe cell apoptosis, oxidative stress and inflammation, associated with increased NF-κB P65 and TNF-α levels as well as decreased myelin associate glycoprotein (MAG) and oligodendrocyte-myelin glycoprotein (OMgp)levels (all <0.01). Compared with the model group, the brain tissues in GCS groups were ameliorated, and cell apoptosis, oxidative stress and inflammation were inhibited, associated with decreased NF-κB P65 and TNF-α levels as well as increased MAG and OMgp levels (all <0.01), which were more markedly in GCS 60 mg/kg group.@*CONCLUSIONS@#GCS can inhibit the NF-κB P65 and TNF-α, reduce the oxidative stress and inflammation, decrease the cell apoptosis in mouse ischemia/reperfusion-induced brain injury model, and 60 mg/kg GCS may be the optimal dose.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Brain
/
Brain Injuries
/
Plant Extracts
/
Random Allocation
/
Chemistry
/
Gene Expression Regulation
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Oxidative Stress
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2019
Type:
Article
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