Single-particle cryo-electron microscopy opens new avenues in structural biology of G protein-coupled receptor / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 39-43, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-775256
ABSTRACT
G protein-coupled receptors(GPCRs)represent the largest class of cell surface receptors,mediating wide range of cellular and physiological processes through their transducers,G proteins and the-arrestins participate in almost all pathological processes. Recent technological advances are revolutionizing the utility of cryo-electron microscopy(cryo-EM),leading to a tremendous progress in the structural studies of biological macromolecules and cryo-EM has played a leading role in the structural biology of GPCR signaling complex. New discoveries of high-resolution threedimensional structures of GPCR signaling complexes based on cryo-EM have emerged vigorously,which depict the common structural characteristics of intermolecular interaction between GPCR and G protein complex-the conformational changes of the transmembrane helix 6 of receptors,and also demonstrate the structural basis of G protein subtype selectivity. Single-particle cryo-EM becomes an efficient tool for identifying the molecular mechanism of receptor-ligand interaction,providing important information for understanding GPCR signaling and the structure-based drug design.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Chemistry
/
Protein Structure, Tertiary
/
Cryoelectron Microscopy
/
Receptors, G-Protein-Coupled
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2019
Type:
Article
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